![]() ![]() A lipase unit was defined as, the amount of enzyme Was determined titrimatrically on the basis of olive oil hydrolysis, as reportedīy Kundu and Pal (1970). The fermented broth was with drawn and centrifuged at 10,000 x g for 15 minĪnd fungal free supernatant was then analyzed for lipase activity. Supplemented with 1.0% (v/v) rapeseed oil at 200 rpm. Technique in shake flasks containing Eggins and Pugh (1962) mineral salts solution This suspension was usedĪs an inoculum to carry out the fermentation process using sub-merged fermentation The spore suspension in distilled water was made with a densityĬontaining 2.5x10 7 spore mL -1. Were grown at 30☑☌ for 96 h on a solid medium composed of potatoĭextrose agar. The stock lab of Institute of Industrial Biotechnology, GC University, Lahore The results were analyzed using commercially available software, Minitab statistical software (2000) for Windows.Īspergillus nigerand Trichoderma viride obtained from A 2 3 full factorial design was employed to optimize the process in shake flasks. In the present research, lipase production was carried out in the combined efforts of Aspergillus niger and Trichoderma viride consortium using Eggins and Pugh mineral salts solution as nutrient source and rapeseed oil as carbon source. However, attempts are being made to over express lipase enzyme to commercially acceptable levels by using more than one strain synergistically. In the last few decades, lipases have received much attention with respect to their potential use in the laundry cleaning for the removal of fatty stains (Savitha et al., 2007).Ī large number of microorganisms have been used for the production of lipases like Fusarium oxysporum, Aspergillus niger, Trichoderma viride and Kluyveromyces lactis (GutieÂrrez et al., 1999 Tamerler and Keshavarz, 2000). Lipases act specifically at oil/water interfaces (Wang et al., 1999 Cihangir and Sarikaya, 2004). Lipases (EC 3.1.1.3) are enzymes, the biological function of which is to catalyze the hydrolysis of tri-acylglycerol to fatty acids, mono- and di-acylglycerols and glycerol. ![]() Research Journal of Microbiology, 2: 639-644. A 2 3 Level Full Factorial Design to Optimize Cultural Conditions for Lipase Production by Consortium of Aspergillus niger and Trichoderma viride. Muhammad Mohsin Javed, Tehmina Saleem Khan and Ikram-ul- Haq, 2007. viride consortium are 30☌ with 1.0% Rapeseed oil as carbon source and medium pH 5.0. Results indicated that optimum conditions for the production of lipase from A. Temperature was also effective but with minor importance than the other two factors. However, the effect of pH was being larger than concentration of Rapeseed oil. Rapeseed oil concentration and pH were found to have positive effects on the production and secretion rate of lipase from consortium. These factors and their optimized levels were analyzed by using 2 3 level factorial designs. The selected three factors were including different concentrations of rapeseed oil (1.0 and 1.5%), pH (5.0 and 6.0) and incubation temperature (25 and 30☌). The effect of three factors on the production rate of lipase was studied. In the presented research, lipase production was carried out using consortium of Aspergillus niger and Trichoderma viride (simultaneously) in shake flasks.
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