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In Adobe Acrobat Pro, Select View > Navigation Panels > Pages. ![]() In this document, a new section has been created with page numberingīeginning with Arabic numeral 1 on the fourth page of the document. Insert ribbon > Page number > Page Number Format Roman numeral page numbering specified In Word using: The image below shows the Word document with lowercase The example document converted from Microsoft Word 2007 has 4 pages, numbered See the list of other software tools in PDF Authoring Tools that Provide Accessibility Support. There are other software tools that perform similar functions. This example is shown with Adobe Acrobat Pro. Examples Example 1: Editing PDF page number formatting specifications using Adobe Acrobat 9 Pro This brings up a dialog box to go to a specific page number. On Windows, this shortcut is "Ctrl + Shift + N" on Mac OS, it is "Cmd + Shift + N". 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For example, Adobe Acrobat Pro and Reader display page numbers in the Page Navigation toolbar. The objective of this technique is to help users locate themselves in a document by ensuring that the page numbering displayed in the PDF viewer page controls has the same page numbering as the document. ![]() ![]() A lipase unit was defined as, the amount of enzyme Was determined titrimatrically on the basis of olive oil hydrolysis, as reportedīy Kundu and Pal (1970). The fermented broth was with drawn and centrifuged at 10,000 x g for 15 minĪnd fungal free supernatant was then analyzed for lipase activity. Supplemented with 1.0% (v/v) rapeseed oil at 200 rpm. Technique in shake flasks containing Eggins and Pugh (1962) mineral salts solution This suspension was usedĪs an inoculum to carry out the fermentation process using sub-merged fermentation The spore suspension in distilled water was made with a densityĬontaining 2.5x10 7 spore mL -1. Were grown at 30☑☌ for 96 h on a solid medium composed of potatoĭextrose agar. The stock lab of Institute of Industrial Biotechnology, GC University, Lahore The results were analyzed using commercially available software, Minitab statistical software (2000) for Windows.Īspergillus nigerand Trichoderma viride obtained from A 2 3 full factorial design was employed to optimize the process in shake flasks. In the present research, lipase production was carried out in the combined efforts of Aspergillus niger and Trichoderma viride consortium using Eggins and Pugh mineral salts solution as nutrient source and rapeseed oil as carbon source. However, attempts are being made to over express lipase enzyme to commercially acceptable levels by using more than one strain synergistically. In the last few decades, lipases have received much attention with respect to their potential use in the laundry cleaning for the removal of fatty stains (Savitha et al., 2007).Ī large number of microorganisms have been used for the production of lipases like Fusarium oxysporum, Aspergillus niger, Trichoderma viride and Kluyveromyces lactis (GutieÂrrez et al., 1999 Tamerler and Keshavarz, 2000). Lipases act specifically at oil/water interfaces (Wang et al., 1999 Cihangir and Sarikaya, 2004). Lipases (EC 3.1.1.3) are enzymes, the biological function of which is to catalyze the hydrolysis of tri-acylglycerol to fatty acids, mono- and di-acylglycerols and glycerol. ![]() Research Journal of Microbiology, 2: 639-644. A 2 3 Level Full Factorial Design to Optimize Cultural Conditions for Lipase Production by Consortium of Aspergillus niger and Trichoderma viride. Muhammad Mohsin Javed, Tehmina Saleem Khan and Ikram-ul- Haq, 2007. viride consortium are 30☌ with 1.0% Rapeseed oil as carbon source and medium pH 5.0. Results indicated that optimum conditions for the production of lipase from A. Temperature was also effective but with minor importance than the other two factors. However, the effect of pH was being larger than concentration of Rapeseed oil. Rapeseed oil concentration and pH were found to have positive effects on the production and secretion rate of lipase from consortium. These factors and their optimized levels were analyzed by using 2 3 level factorial designs. The selected three factors were including different concentrations of rapeseed oil (1.0 and 1.5%), pH (5.0 and 6.0) and incubation temperature (25 and 30☌). The effect of three factors on the production rate of lipase was studied. In the presented research, lipase production was carried out using consortium of Aspergillus niger and Trichoderma viride (simultaneously) in shake flasks. ![]() New zone, new scenery: now we’re in what appears to be a solarium with a night view full of stars. Once you’ve nabbed all the carrots in here, it’s time to move onto Zone 3. On Level 7 especially, towards the end, make sure you jump to the edge of the right side to get those last carrots. Starting in Zone 2, Level 5, you’ll also encounter a new platform: a Carrot-pult, moving platform hybrid!Īs you go through each level, make sure to keep an eye out on the mini-map to know where all the carrots are so you can plan your jumps in the right directions. So far, the door has tended to be on the bottom-right corner, but starting now you’ll find it occasionally in the top-right. Hare must have been cooking up all his carrots and evil schemes! Moving zones brings about a change in scenery: now we’re in a kitchen area, the place where Dr. Once you jump and bounce your way through the 10 levels of Zone 1, you’ll be ready to take on Zone 2! Zone 2: Recipe for Disaster Sometimes it’s a good idea to wait for a platform so you can use it to take you somewhere. These can take you across from one point to another, and they keep moving back and forth. Zone 1, Level 7 is where you’ll start to encounter moving platforms. Notice the green platforms too: you can go through them when you’re jumping up, but once you’re standing on them, you can’t go down through them.Īt Zone 1, Level 6, you’ll be introduced to the Carrot-pult: a big pink bouncy blob that will bounce you up, allowing you to reach up higher to grab more carrots. Here’s a labeled screenshot to show where things are: If you’re running low on time, the timer (at the top) will turn red and start making low beeping sounds. As long as you make it through that door before time runs out, you’re good to go. Once you get them all, the door to the next level will unlock. The game starts out pretty straightforward: you just run and collect the carrots. Zone 1: Baby Carrot StepsĪs with all the zones, you’ll be faced with a series of doors, which you can only unlock one after the other as you defeat each round. Now continue to the right and enter Zone 1 to begin playing. |
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